交联柚苷酶聚集体水解柚皮苷制备柚皮素Hydrolysis of naringin to naringeninby cross-linked naringinase aggregates
朱必玉;钱伟;王先锋;杜平;高乾坤;魏胜华;
摘要(Abstract)：
为了提高柚皮素的产率,简化分离提纯的工艺,得到较高纯度的柚皮素,采用交联柚苷酶聚集体水解柚皮苷制备柚皮素,以生成的柚皮素质量浓度和得率为优化指标.研究了底物质量浓度、温度、pH、加酶量、反应时间对制备柚皮素的影响,利用正交设计对水解过程进行优化,确定最佳工艺条件为底物质量浓度4.0g/L、温度55℃、pH 3.0、加酶量18.0mg/mL、反应时间24h.优化后制备得到的柚皮素质量浓度为1.833g/L,柚皮素得率为97.72%,为制备柚皮素提供了一种新的方法.
关键词(KeyWords)： 交联柚苷酶聚集体;柚皮素;水解反应;正交试验
基金项目(Foundation): 国家级大学生创新创业训练计划基金资助项目(201310363075)
作者(Author): 朱必玉;钱伟;王先锋;杜平;高乾坤;魏胜华;
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参考文献(References)：
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